Our goal is to understand, model, and recapitulate in vitro the instructive signals utilized by human embryos to pattern tissue-specific differentiation of pluripotent stem cells, and apply this knowledge towards the rational design of tissue engineered scaffolds and other regenerative therapeutic strategies. Currently, we primarily focus on generating tissues and therapies for the central nervous system.
Meet the Lab with WI PBS!
"Can you help me make a neural tube?" SCBRM Nervous System Engineers engage in Educational Outreach!
Dr. Ashton presents the business plan of Neurosetta, LLC at SUMMERFEST Tech WARF Innovation Day in Milwaukee June 29th, 2022.
Neural Rosette Cutaway
Micro-patterned substrate tech allows for single neural rosette formation. Green (Pax6), Red (B3 tubulin), Blue (Nuclear).
Dr. Randolph Ashton discusses the importance of scientific research with WI Governor Tony Evers and Lt. Governor Mandela Barnes!
HOX Patterning Precision
Precisely control NSC regionalization along the hindbrain and spinal cord’s R/C axis (governed by expression of 39 Hox genes).
Exploring New Questions
Postdoctoral Researcher Nisha Iyer shows Undergraduate Researcher Assistant Stephanie Cuskey an RNA Isolation reagent.
2D neural rosettes grown in cell culture. N-Cadherin (red); Cell nuclei (blue); Laminin (green).
Postdoctoral Researcher Gavin Knight presenting his research at the 12th Annual Wisconsin Stem Cell Symposium.
The E6 PROTOCOL
The simplest and fastest protocol for differentiating hPSCs into a pure neural cell culture, xeno-free & clinically translatable.
Dr. Alireza Aghayee watching former undergraduate researcher Lexi Doersch practice a cell culture technique.
Dr. Carlos Marti-Figueroa & Graduate Research Assistant Frank Seipel inspect a fresh section of organoid tissue on a glass slide.
SCRMC Fellowship Award
Dr. Nisha Iyer (right) receiving a SCRMC Postdoctoral Fellowship Award from Prof. William Murphy (left) & Prof. Tim Kamp (middle)!