Advanced culture substrate engineering using simple reactions. The SCBRM lab has discovered a novel reaction scheme that uses simple nucleophilic substitution reactions coupled with ‘click’ chemistry to enable fabrication of cell culture substrates presenting multiple orthogonal biological entities in discrete microarray orientations. Using this method, we can engineer culture substrates capable of facilitating ‘biospecific’ interactions to parse the singular and combinatorial effects of immobilized biological ligands at various surface densities on cell fate. We plan to use such substrates to elucidate how immobilized biological cues can be used to precisely control the fate of human pluripotent stem cells or their neural derivatives in a spatiotemporal manner. For more information, check out our Biomacromolecules publication. Schematic generated by Jin Sha.