University of Wisconsin–Madison

Publications

23. Deriving, Regenerating, and Engineering CNS Tissues using Human Pluripotent Stem Cells
K Lemke, A Aghayee, RS Ashton (2017)
Current Opinion in Biotechnology [doi:10.1016/j.copbio.2017.05.010]

22. The Case for Applying Tissue Engineering Methodologies to Instruct Human Organoid Morphogenesis
CR Marti-Figueroa, RS Ashton (2017)
Acta Biomaterialia [doi:10.1016/j.actbio.2017.03.023]

21. Chemically defined differentiation of human pluripotent stem cells to hindbrain and spinal cord neural stem cells with defined regional identities
ES Lippmann, MC Estevez-Silva, RS Ashton (2015)
Protocol Exchange [doi:10.1038/protex.2015.076]

20. High-content imaging with micropatterned multiwell plates reveals influence of cell geometry and cytoskeleton on chromatin dynamics
Harkness. T., McNulty, J.D., Prestil, R., Seymour, S.K., Klann, T., Murrell, M., Ashton, R. S. and Saha, K. (2015)
Biotechnology Journal. Epub. [PMID: 26097126]

19. Deterministic HOX patterning in human pluripotent stem cell-derived neuroectoderm
ES Lippmann, CE Williams, DA Ruhl, MC Estevez-Silva, ER Chapman, JJ Coon, RS Ashton (2015)
Stem Cell Reports, 4(4): 632-44

18. Micropatterned, clickable culture substrates enable in situ spatiotemporal control of human PSC-derived neural tissue morphology
Knight GT, Sha J, Ashton RS (2015)
Chemical Communications, 51(25): 5238-5241

17. Multifunctional drug nano carriers formed by cRGD-conjugated βCD-PAMAM-PEG for targeted cancer therapy
Saraswathy M, Knight GT, Pilla S, Ashton RS, Gong S (2015)
Colloids and Surfaces B: Biointerfaces 126: 590-97

16. Fabricating Complex Culture Substrates Using Robotic Microcontact Printing (R-µCP) and Sequential Nucleophilic Substitution
Knight GT, Klann T, McNulty JS, Ashton RS (2014)
J Vis Exp (92): e52186 

15. High-precision robotic microcontact printing (R-µCP) utilizing a vision guide selectively compliant articulated robotic arm
McNulty JD, Klann T, Sha J, Salick M, Knight GT, Turng L, Ashton RS (2014)
Lab Chip, 14(11): 1923-30

14. Micropattern width dependent sarcomere development in human ESC-derived cardiomyocytes
Salick M, Napiwocki BN, Sha J, Knight GT, Chindhy SA, Kamp TJ, Ashton RS, Crone WC (2014)
Biomaterials, 35(3): 4454-64

13. Defined human pluripotent stem cell culture enables highly efficient neuroepithelium derivation independent of small molecule inhibitors
Lippmann ES, Estevez-Silva MC, Ashton RS (2014)
Stem Cells, 32(4): 1032-42

12. Multivalent sonic hedgehog enhances differentiation of human embryonic stem cells into dopaminergic and GABAergic neurons
Vazin T, Ashton RS, Conway A, Rode N, Lee SM, Bravo V, Healy KE, Kane R, Schaffer DV (2014)
Biomaterials, 35(3): 941-8

11. Sequential nucleophilic substitutions permit orthogonal click functionalization of multicomponent PEG brushes
Sha J, Lippmann ES, McNulty J, Ma Y, Ashton RS (2013)
Biomacromolecules, 14(9): 3294-303

10. Astrocytes regulate adult hippocampal neurogenesis through ephrin-B signaling
Ashton RS, Nat Neurosci, Conway A, Pangakar C, Bergen J, Lim K, Shah P, Bissell M, Schaffer DV (2012)
Nat Neurosci, 15(10): 1399-406

9. Molecular characterization of multivalent bioconjugates by size-exclusion chromatography with multiangle laser light scattering
Pollock JF, Ashton RS, Rode NA, Schaffer DV, Healy KE (2012)
Bioconjugate Chem, 23(9): 1794-801

8. Progress and prospects in stem cell engineering
Ashton RS, Keung AJ, Peltier J, Schaffer DV (2011)
Annual Rev Chem Biomol Eng, 2: 479-502

7. The influence of hydrogel modulus on the proliferation and differentiation of encapsulated neural stem cells
Banerjee, A., Arha, M., Choudhary, S., Ashton, R.S., Bhatia, S.R., Schaffer, D.V., Kane, R.S. (2009)
Biomaterials, 27: 4695-99

6. Multivalency of sonic hedgehog conjugated to linear polymer chains modulates protein potency
Wall ST, Saha KS, Ashton RS, Kim K, Schaffer DV, Healy KE (2008)
Bioconjugate Chem. 19: 306-12

5. Scaffolds based on degradable alginate hydrogels and poly(lactide-co-glycolide) microspheres for stem cell culture
Ashton RS, Banerjee A, Punyani S, Schaffer DV, Kane RS (2007)
Biomaterials, 28: 5518-25

4. High-throughput screening of gene function in stem cells using clonal microarrays
Ashton RS, Peltier J, Fasano CA, O’Neill A, Leonard J, Temple S, Schaffer DV, Kane RS (2007)
Stem Cells, 25: 2928-35

3. Raftlike polyvalent inhibitors of anthrax toxin: modulating inhibitory potency by formation of lipid microdomains
Rai PR, Saraph A, Ashton R, Poon V, Mogridge J, Kane RS (2007)
Angew Chem Int Ed Engl. 46: 2207-09

2. Thiol-mediated anchoring of ligands to self-assembled monolayers for studies of biospecific interactions
Gujraty KV, Ashton R, Bethi SR, Kate S, Faulkner CJ, Jennings GK, Kane RS (2006)
Langmuir 22:10157-62

1. Microfluidic separation of DNA
Ashton R, Padala C, and Kane RS (2003)
Curr Opin Biotechnol, 14: 497-504