Our goal is to understand, model, and recapitulate in vitro the instructive signals utilized by human embryos to pattern tissue-specific differentiation of pluripotent stem cells, and apply this knowledge towards the rational design of tissue engineered scaffolds and other regenerative therapeutic strategies. Currently, we primarily focus on generating tissues and therapies for the central nervous system.
Neural Rosette Cutaway
Micro-patterned substrate tech allows for single neural rosette formation. Green (Pax6), Red (B3 tubulin), Blue (Nuclear).
SCRMC Fellowship Award
Dr. Nisha Iyer (right) receiving a SCRMC Postdoctoral Fellowship Award from Prof. William Murphy (left) & Prof. Tim Kamp (middle)!
Maria presenting "Towards an In Vitro Model of the Human Posterior CNS" at ISSCR 2017, the world's premier stem cell conference.
HOX Patterning Precision
Precisely control NSC regionalization along the hindbrain and spinal cord’s R/C axis (governed by expression of 39 Hox genes).
Graduate student Gavin Knight presenting his research at 12th Annual Wisconsin Stem Cell Symposium.
2D neural rosettes grown in cell culture. N-Cadherin (red); Cell nuclei (blue); Laminin (green).
Graduate student Alireza Aghayee instructing new undergraduate researcher Lexi Doersch with a new experimental practice.
Graduate student Carlos Marti-Figueroa and Undergraduate Researcher Frank Seipel look at a fresh section of organoid tissue.
The E6 PROTOCOL
The simplest and fastest protocol for differentiating hPSCs into a pure neural cell culture, xeno-free & clinically translatable.
Exploring New Questions
Post Doctoral Researcher Nisha Iyer shows Undergraduate Researcher Stephanie Cuskey an RNA Isolation reagent.
Dr. Ashton, Stephanie, and Nikolai viewing a rat spinal cord section using a manual microscope.