University of Wisconsin–Madison

Neural Rosettes


Using our E6 protocol published in Stem Cells, we can differentiate human pluripotent stem cells into the neural stem cells that give rise to your brain and spinal cord. Similar to their actions in vivo during development, these neural stem cells polarize in 2D culture (i.e. polarized structures called ‘rosettes’) in an attempt to form the early neural tube structures from which your brain and spinal cord are generated. Also similar to the developing neural tube, cells in the rosettes deposit extracellular matrix proteins in a polarized fashion.